In typical complementary DNA microarray experiments, two fluorescently labeled RNAs are hybridized to an array of cDNA probes on a glass slide, and their relative gene expression intensities, ratios ...
A microarray experiment involves several steps, including spotting complementary DNA, extracting RNA, labeling the probe, hybridizing, scanning and analyzing images. Each step introduces variability, ...
The bound cDNA indicates a gene that is expressed because the complimentary partner strands are already within the microarray. Any unbound cDNA can be washed off the microarray so only the bound, ...
In microarray gene expression experiments, several diverse conditions in each experiment (e.g., time, doses, replicates) increase the data points from tens of thousands of measurements for a single ...
Microarrays are a two-dimensional arrangement of biological samples, with samples placed in spots numbering in the thousands and organized into columns and rows. They allow for efficient analysis of ...