The ability to site-selectively incorporate a synthetic moiety like a biophysical probe, an unnatural amino acid, or a defined posttranslational modification into a protein of interest opens up a ...

Why do problems occur with a special variant of 'protein glues', the split inteins, that severely limit their use in producing proteins? A team has now answered this question. Proteins are the ...

Protein splicing is a self-catalysed post-translational process in which an intervening polypeptide segment, known as an intein, excises itself from a precursor protein and ligates the flanking ...

Several methods have been described recently that permit researchers to exert post-translational control over gene products. An intein-based method shows it is a strong contender in the field by ...

The SCN1A gene is too large to fit into aconventional viral delivery systems. Scientists overcame this hurdle with a pioneering "split-intein" mechanism, which is like two separate trucks, delivering ...

Proteins are the building blocks of life. They consist of folded peptide chains, which in turn are made up of a series of amino acids. From stabilising cell structure to catalysing chemical reactions, ...

Long-standing problem solved: Researchers discover that misfolding prevents efficient utilisation of so-called split inteins as ‘protein glue’ Proteins are the building blocks of life. They consist of ...