Hi-C (genomic analysis technique) - Wikipedia
WEBThe outline of the classical Hi-C workflow is as follows: cells are cross-linked with formaldehyde; chromatin is digested with a restriction enzyme that generates a 5’ overhang; the 5’ overhang is filled with biotinylated bases and the …
The Hitchhiker's Guide to Hi-C Analysis: Practical guidelines
WEBThe aim of this paper is to provide guidelines for analyzing and interpreting data obtained with genome-wide 3C methods such as Hi-C and 3C-seq that rely on deep sequencing to detect and quantify pairwise chromatin interactions genome-wide. Go to: Introduction.
Introduction to Hydrophobic Interaction Chromatography - Bio-Rad
WEBHydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity. HIC is a useful separation technique for purifying proteins while maintaining biological activity due to the use of conditions and matrices that operate under less denaturing conditions.
GrapHi-C: graph-based visualization of Hi-C datasets
WEBJun 29, 2018 · Hi-C is a proximity-based ligation reaction used to detect regions of the genome that are close in 3D space (or “interacting”). Typically, results from Hi-C experiments (contact maps) are visualized as heatmaps or Circos plots.
Hydrophobic Interaction Chromatography | Cytiva
WEBHydrophobic interaction chromatography (HIC) separates proteins according to differences in their surface hydrophobicity. HIC utilizes a reversible interaction between the proteins and the hydrophobic ligand of a HIC resin.
HiC-bench: comprehensive and reproducible Hi-C data analysis …
WEBJan 5, 2017 · HiC-bench focuses on the reproducibility of the analysis by keeping records of the source code, tool versions and parameter settings, and it is the only HiC-analysis pipeline that allows combinatorial parameter exploration facilitating …
Hydrophobic Interaction Chromatography: Understanding its …
WEBJan 10, 2024 · What is hydrophobic interaction chromatography? Hydrophobic Interaction Chromatography (HIC) is a technique used in biochemistry and analytical chemistry to separate and purify proteins based on their hydrophobic properties.
MHiC, an integrated user-friendly tool for the identification and ...
WEBMar 12, 2020 · Hi-C is a molecular biology technique to understand the genome spatial structure. However, data obtained from Hi-C experiments is biased. Therefore, several methods have been developed to model Hi-C data and identify significant interactions.
Guide to Hi-C & Related Methods - Active Motif
WEBApr 20, 2020 · Hi-C. These early 3C-based methods culminated in the development of Hi-C in 2009, providing an “all-vs-all” approach for genome-wide identification of chromatin interactions. As with its predecessors, Hi-C begins with …
HiC-bench: comprehensive and reproducible Hi-C data analysis …
WEBJan 5, 2017 · HiC-bench performs all common Hi-C analysis tasks, such as alignment, filtering, contact matrix generation and normalization, identification of topological domains, scoring and annotation of specific interactions using both published tools and our own.